Journal:

Article Title: Ruminococcin A, a New Lantibiotic Produced by a Ruminococcus gnavu s Strain Isolated from Human Feces

doi: 10.1128/AEM.67.9.4111-4118.2001

Figure Lengend Snippet: Bacterial strains: sensitivity to RumA and metronidazole a

Article Snippet: TABLE 1 Species Strain Culture medium MIC (μg/ml) of agent: Prepurified RumA Metronidazole Bacteroides putredenis ATCC 29800 BHI-YH NI ∗ 3.0 Bacteroides thetaiotaomicron ATCC 29148 BHI-YH NI ∗ 12.5 Bacteroides vulgatus ATCC 8482 BHI-YH 600 12.5 Bacteroides fragilis ATCC 25285 BHI-YH NI ∗ NI ∗∗ Bifidobacterium adolescentis ATCC 15703 BHI-YH 150 NI ∗∗ Bifidobacterium breve ATCC 15700 BHI-YH 300 NI ∗∗ Bifidobacterium catenulatum ATCC 27539 BHI-YH 150 NI ∗∗ B. longum ATCC 15707 BHI-YH 75 NI ∗∗ Enterococcus faecalis CIP 76117 BHI-YH NI ∗ NT Eubacterium contortum ATCC 25540 BHI-YH 32.5 1.5 Ruminococcus obeum ATCC 29174 BHI-YH 32.5 1.5 R. torques ATCC 27756 BHI-YH 300 NT R. gnavus ATCC 29149 BHI-YH 75 12.5 C. nexile ATCC 27757 BHI-YH 150 3.0 C. oroticum ATCC 13619 BHI-YH 600 6.25 C. perfringens CpA BHI-YH 75 25 C. difficile serogroup A B-1 BHI-YH 75 6.25 C. difficile serogroup C C253 BHI-YH 75 6.25 C. difficile serogroup D ATCC 43598 BHI-YH 150 6.25 C. difficile serogroup F M-1 BHI-YH 150 12.5 C. difficile serogroup S3 79685 BHI-YH 75 6.25 C. difficile ATCC 43255 BHI-YH 150 6.25 C. botulinum type A CIP 38 TGY 75 NT C. botulinum type B NCTC 7273 TGY 75 NT C. botulinum type E NCTC 8266 TGY 75 NT C. sordellii VPI 9048 BHI-YH 150 NT C. bifermentans NCTC 506 BHI-YH 75 NT C. septicum ATCC 12464 BHI-YH 150 NT C. sporogenes ATCC 19404 BHI-YH 300 NT B. cereus Z4222, TZ415 J agar 75 NT B. cereus Z4234, K1231, P2101 J agar 150 NT B. cereus K1166, L2104, P2174, Z421, P21S J agar NI ∗ NT Open in a separate window a In this test, a Sep-pack-purified RumA containing 600 μg of total protein per ml was used.

Techniques:

Journal: bioRxiv

Article Title: Gut microbiota-derived tryptamine impairs insulin sensitivity

doi: 10.1101/2022.03.05.483098

Figure Lengend Snippet: In vivo tryptamine production by T2D-associated tryptamine producers and engineered gut microbe impairs insulin signaling. (A) Fecal tryptamine level in mice from control group (n=6) and antibiotics mixture treatment (ABX) group (n=12). (B-D) Fecal tryptamine level, oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) indexes in antibiotics mixture (ABX)-treated mice following colonization of either R. gnavus ATCC 29149 and i.p. administration of TAAR1 antagonist EPPTB (10mg/kg) (n=6 per group). * comparisons between control group and R. gnavus group. # comparisons between R. gnavus group and R. gnavus group + TAAR1 antagonist (EPPTB) group. (E) Western blot (and semi-quantification) of R. gnavus ATCC 29149 and EPPTB administration treatment on Akt activation simulated by insulin (1U/kg) in white adipose tissue (WAT) lysates from pseudo germ-free mice (n=2-3 per group). (F-H) Fecal tryptamine level, oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) indexes in antibiotics mixture (ABX)-treated mice following colonization of either engineered L. casei TDC+ or L. casei with empty vector (n=6 per group). (I) Western blot (and semi-quantification) of colonization of either engineered L. casei TDC+ or L. casei with empty vector on Akt activation simulated by insulin (1U/kg) in white adipose tissue (WAT) lysates from pseudo germ-free mice (n=2-3 per group). *, # p < 0.05, **,## p < 0.01 and ***, ### p < 0.001. P values were determined by ordinary one-way ANOVA or Student’s t-test. See also .

Article Snippet: S5), we demonstrated R. gnavus (ATCC 29149) is a T2D-associated tryptamine-producing bacteria strain.

Techniques: In Vivo, Control, Western Blot, Activation Assay, Plasmid Preparation

Journal: bioRxiv

Article Title: Gut microbiota-derived tryptamine impairs insulin sensitivity

doi: 10.1101/2022.03.05.483098

Figure Lengend Snippet: In vivo tryptamine production by T2D-associated tryptamine producers and engineered gut microbe impairs insulin signaling. (A) Tryptamine level in culture medium of R. gnavus ATCC 29149. (B) Tryptamine level in culture medium of L. casei TDC+ and L. casei vector control.

Article Snippet: S5), we demonstrated R. gnavus (ATCC 29149) is a T2D-associated tryptamine-producing bacteria strain.

Techniques: In Vivo, Plasmid Preparation, Control